Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid-nitrogen temperatures). Cryo-EM is gaining popularity in structural biology. The utility of transmission electron cryomicroscopy stems from the fact that it allows the observation of specimens that have not been stained or fixed in any way, showing them in their native environment. This is in contrast to X-ray crystallography, which requires crystallizing the specimen, which can be difficult, and placing them in non-physiological environments, which can occasionally lead to functionally irrelevant conformational changes. Advances in electron detector technology, particularly DED (Direct Electron Detectors) as well as more powerful software imaging algorithms have allowed for the determination of macromolecular structures at near-atomic resolution. Imaged macromolecules include viruses, ribosomes, mitochondria, ion channels, and enzyme complexes. Starting in 2018, cryo-EM could applied to structures as small as hemoglobin (64 kDa) and with resolutions up to 1.8 Å. In 2019, cryo-EM structures represented 2.5% of structures deposited in the Protein Data Bank, and this number continues to grow. An application of cryo-EM is cryo-electron tomography (cryo-ET), where a 3D reconstruction of the sample is created from tilted 2D images. (Wikipedia).
Chris Russo - Potential electron cryomicroscopy in situ: technology to identify molecules in cells
Recorded 15 November 2022. Chris Russo of the University of Cambridge presents "The potential of electron cryomicroscopy in situ: new technology to identify molecules in cells" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: Electron cryomicroscopy (cryoEM) of biological
From playlist 2022 Cryo-Electron Microscopy and Beyond
Petar Petrov - Laser phase-contrast transmission electron microscopy & computational opportunities
Recorded 15 November 2022. Petar Petrov of the University of California, Berkeley, presents "Laser phase-contrast transmission electron microscopy and associated computational opportunities" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: A phase plate can provide optimum
From playlist 2022 Cryo-Electron Microscopy and Beyond
Mathematics for cryo-electron microscopy – Amit Singer – ICM2018
Mathematics in Science and Technology Invited Lecture 17.8 Mathematics for cryo-electron microscopy Amit Singer Abstract: Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biologica
From playlist Mathematics in Science and Technology
Cryogenic Engines | The complete physics
Let's understand the detailed working of cryogenic engines in a logical manner. Be our supporter or contributor: https://www.youtube.com/channel/UCqZQJ4600a9wIfMPbYc60OQ/join instagram : https://www.instagram.com/sabinzmathew/ Twitter : https://twitter.com/sabinsmathew Telegram : https://
From playlist Aerospace & Aeronautical
Steven Ludtke - Contextual Conformational Variability in CryoEM and CryoET using Deep Learning
Recorded 14 November 2022. Steven Ludtke of Baylor College of Medicine presents "Contextual Conformational Variability in CryoEM and CryoET using Deep Learning" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: By studying molecules in solution, free to explore their entrop
From playlist 2022 Cryo-Electron Microscopy and Beyond
Diamagnetic Anisotropy - H NMR Spectroscopy - Organic Chemistry
This organic chemistry video tutorial provides a basic introduction into the concept of diamagnetic anisotropy as it relates to H NMR spectroscopy. It explains why the chemical shift of molecules such as benzene and ethylene are much higher than for those of acetylene. It also uses 18-An
From playlist New Organic Chemistry Playlist
Serena Yeung - Computer Vision for Segmenting & Analyzing Subcellular Components in CE Tomography
Recorded 15 November 2022. Serena Yeung of Stanford University presents "Developing Computer Vision Methods for Segmenting and Analyzing Subcellular Components in Cryo-Electron Tomography" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: CryoET technology is generating sig
From playlist 2022 Cryo-Electron Microscopy and Beyond
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From playlist Biology
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New advances in electron microscopy reveal molecular structures at resolutions useful for drug discovery. Produced by Science and the National Cancer Institute. Animation Credit: Veronica Falconieri and Sriram Subramaniam/LCB/CCR/NCI/NIH Link to article: http://scim.ag/1dYcNi0
From playlist Materials and technology
Carlos Esteve Yague - Spectral decomposition of atomic structures in heterogeneous cryo-EM
Recorded 18 November 2022. Carlos Esteve-Yague of the University of Cambridge Department of Applied Mathematics and Theoretical Physics presents "Spectral decomposition of atomic structures in heterogeneous cryo-EM" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: In this
From playlist 2022 Cryo-Electron Microscopy and Beyond
Photonic and Electronic Properties in Quantum Dot Circuit-QED Systems by Bijay Kumar Agarwalla
PROGRAM NON-HERMITIAN PHYSICS (ONLINE) ORGANIZERS: Manas Kulkarni (ICTS, India) and Bhabani Prasad Mandal (Banaras Hindu University, India) DATE: 22 March 2021 to 26 March 2021 VENUE: Online Non-Hermitian Systems / Open Quantum Systems are not only of fundamental interest in physics a
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Quantum Transport, Lecture 6: Quantum Point Contacts II
Instructor: Sergey Frolov, University of Pittsburgh, Spring 2013 http://sergeyfrolov.wordpress.com/ Summary: Advanced topics related to one-dimensional quantum transport. 1) Non-linear regime in Quantum Point Contacts 2) Landauer-Buttiker formalism 3) Spin filtering properties of Quantum P
From playlist Quantum Transport
Quantum Transport, Lecture 8: Quantum Dots
Instructor: Sergey Frolov, University of Pittsburgh, Spring 2013 http://sergeyfrolov.wordpress.com/ Summary: Basics of transport through quantum dots are discussed, with a focus on quantum confinement energy and orbital excited states. Lecture also introduces double and triple quantum dots
From playlist Quantum Transport
The Quantum Barrier Potential Part 2: Defining the Transmission and Reflection Coefficients
In the previous tutorial we introduced our second quantum problem, that of the quantum barrier potential. Again, this involves a free particle that encounters a barrier of finite potential. We've already solved the Schrödinger equation for this system, so let's now use those results to det
From playlist Modern Physics
Physics - Ch 66 Ch 4 Quantum Mechanics: Schrodinger Eqn (89 of 92) Which Penetrates Easier?
Visit http://ilectureonline.com for more math and science lectures! In this video I will compare the transmission coefficients and the probability of an electron and proton of the same energy making through the barrier, where the electron and proton are at an energy level E=10eV and the i
From playlist PHYSICS 66.1 QUANTUM MECHANICS - SCHRODINGER EQUATION
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Nanostructures and Nanomaterials: Characterization and Properties by Characterization and Properties by Dr. Kantesh Balani & Dr. Anandh Subramaniam,Department of Nanotechnology,IIT Kanpur.For more details on NPTEL visit http://nptel.ac.in.
From playlist IIT Kanpur: Nanostructures and Nanomaterials | CosmoLearning.org
(January 27, 2010) Suedeen Kelly, former commissioner with the Federal Energy Regulatory Commission, discusses from a regulatory perspective the multi-layered complexities in reforming the large-scale electricity distribution infrastructure in the United States and provides suggestions for
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Joe Kileel - Method of moments in cryo-EM - IPAM at UCLA
Recorded 16 November 2022. Joe Kileel of the University of Texas at Austin presents "Method of moments in cryo-EM" at IPAM's Cryo-Electron Microscopy and Beyond Workshop. Abstract: In this talk, I will present recent advances in the theory and implementation of method of moments-based appr
From playlist 2022 Cryo-Electron Microscopy and Beyond
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Nanostructures and Nanomaterials: Characterization and Properties by Characterization and Properties by Dr. Kantesh Balani & Dr. Anandh Subramaniam,Department of Nanotechnology,IIT Kanpur.For more details on NPTEL visit http://nptel.ac.in.
From playlist IIT Kanpur: Nanostructures and Nanomaterials | CosmoLearning.org