Biochemistry

  1. Techniques in Biochemistry
    1. Chromatography
      1. Ion Exchange Chromatography
        1. Principle and Mechanism
          1. Types of Ion Exchangers
            1. Cation Exchangers
              1. Anion Exchangers
              2. Applications in Protein Purification
              3. Gel Filtration Chromatography
                1. Principle and Mechanism
                  1. Molecular Weight Estimation
                    1. Applications in Protein Separation
                    2. Affinity Chromatography
                      1. Principle and Mechanism
                        1. Types of Affinity Ligands
                          1. Immunoaffinity
                            1. Metal Chelate Affinity
                            2. High-specificity Binding Interactions
                          2. Electrophoresis
                            1. SDS-PAGE
                              1. Principle and Mechanism
                                1. Gel Preparation and Sample Loading
                                  1. Protein Staining and Visualization
                                    1. Applications in Protein Analysis
                                    2. Agarose Gel Electrophoresis
                                      1. Principle and Mechanism
                                        1. Agarose Concentration and Gel Casting
                                          1. DNA Fragment Separation
                                            1. Applications in Nucleic Acid Analysis
                                          2. Spectroscopy
                                            1. UV-Vis Spectroscopy
                                              1. Principle and Mechanism
                                                1. Absorption of Light by Biomolecules
                                                  1. Applications in Concentration Determination
                                                  2. Fluorescence Spectroscopy
                                                    1. Principle and Mechanism
                                                      1. Fluorescent Labeling Techniques
                                                        1. Applications in Protein Studies and Cellular Imaging
                                                        2. NMR Spectroscopy
                                                          1. Principle and Mechanism
                                                            1. Chemical Shift and Coupling Constants
                                                              1. Applications in Structural Biology and Metabolite Analysis
                                                            2. Mass Spectrometry
                                                              1. Principle and Mechanism
                                                                1. Ionization Techniques
                                                                  1. Electrospray Ionization (ESI)
                                                                    1. Matrix-Assisted Laser Desorption/Ionization (MALDI)
                                                                    2. Applications in Protein Identification and Quantification
                                                                    3. X-Ray Crystallography
                                                                      1. Principle and Mechanism
                                                                        1. Crystal Growth Techniques
                                                                          1. Data Collection and Structure Determination
                                                                            1. Applications in Determining 3D Structures of Proteins
                                                                            2. Western Blotting
                                                                              1. Principle and Mechanism
                                                                                1. Transfer of Proteins to Membranes
                                                                                  1. Antibody Probing and Detection
                                                                                    1. Applications in Protein Identification and Quantification
                                                                                    2. ELISA
                                                                                      1. Principle and Mechanism
                                                                                        1. Types of ELISA
                                                                                          1. Direct ELISA
                                                                                            1. Indirect ELISA
                                                                                              1. Sandwich ELISA
                                                                                              2. Applications in Quantifying Hormones, Proteins, and Antibodies
                                                                                            4. Cellular Processes
                                                                                            First Page
                                                                                            6. Applications of Biochemistry