An imaging cycler microscope (ICM) is a fully automated (epi)fluorescence microscope which overcomes the spectral resolution limit resulting in parameter- and dimension-unlimited fluorescence imaging. The principle and robotic device was described by Walter Schubert in 1997 and has been further developed with his co-workers within the human toponome project. The ICM runs robotically controlled repetitive incubation-imaging-bleaching cycles with dye-conjugated probe libraries recognizing target structures in situ (biomolecules in fixed cells or tissue sections). This results in the transmission of a randomly large number of distinct biological informations by re-using the same fluorescence channel after bleaching for the transmission of another biological information using the same dye which is conjugated to another specific probe, a.s.o. Thereby noise-reduced quasi-multichannel fluorescence images with reproducible physical, geometrical, and biophysical stabilities are generated. The resulting power of combinatorial molecular discrimination (PCMD) per data point is given by 65,536k, where 65,536 is the number of grey value levels (output of a 16-bit CCD camera), and k is the number of co-mapped biomolecules and/or subdomains per biomolecule(s). High PCMD has been shown for k = 100, and in principle can be expanded for much higher numbers of k. In contrast to traditional multichannel–few-parameter fluorescence microscopy (panel a in the figure) high PCMDs in an ICM lead to high functional and spatial resolution (panel b in the figure). Systematic ICM analysis of biological systems reveals the supramolecular segregation law that describes the principle of order of large, hierarchically organized biomolecular networks in situ (toponome). The ICM is the core technology for the systematic mapping of the complete protein network code in tissues (human toponome project). The original ICM method includes any modification of the bleaching step. Corresponding modifications have been reported for antibody retrieval and chemical dye-quenching debated recently. The Toponome Imaging Systems (TIS) and multi-epitope-ligand cartographs (MELC) represent different stages of the ICM technological development. Imaging cycler microscopy received the American ISAC best paper award in 2008 for the three symbol code of organized proteomes. (Wikipedia).
Live from the ATLAS Experiment
Find out more about the ATLAS Experiment and recent upgrades of the detector.
From playlist 360° videos
DIY Scanning Electron Microscope - Overview
Today, I finally produced an image with my DIY scanning electron microscope. I've spent the last few months working on this project, and am encouraged by today's success. There is still a lot of work left to do in making the image higher resolution, and eliminating sources of noise, howe
From playlist Scanning Electron Microscope
DIY Scanning Electron Microscope - Operation procedure
After getting back from Maker Faire (which is always a hugely enjoyable and inspiring event), I thought that my microscope might need some repairs. As it turned out, I only had to change the filament and tighten some screws that came loose during the trip back from the Faire. The microsc
From playlist Scanning Electron Microscope
Frequency response test for thermal imaging sensor
I am testing the frequency response of the TPS334 IR sensor. My very crude tests agree with the datasheet, which means that the image scan time will be very long for high contrast images. Many low-cost microbolometer-based thermal imaging cameras have 80x80 sensors (6400 pixels). At a 1
From playlist Electronics
http://www.nucleushealth.com/ - This 3D medical animation shows the anatomy and physiology of skin and demonstrates an excisional biopsy a surgical techniques commonly used to obtain a sample tissue of suspected skin disorder. A skin biopsy is used to view under a microscope and make a dia
From playlist Healthcare Patient Education Animations
Fluorescent microscopes are amazing!
Fluorescent microscopy was always one of my favorite parts of working with mammalian cells as it always made for spectacular images. There are a variety of techniques that make use of fluorescence microscopy, many of which are used regularly in the best labs in the world. But fluorescence
From playlist Biology and Genetics
Immunofluorescent Imaging, Nanoparticle Systems and Preparing NHS Fluorescein
Since the invention of the microscope, we've been looking for better and better ways to see into the microscopic world. At first we used simple dyes to colour particular parts of a cell to make the pieces more clear. But as time went on we demanded something better. Enter immunofluorescent
From playlist Biology and Genetics
I'm putting this here for a talk I'm giving next week. It is how we pump our nanoparticle samples for optical measurements. I'm sure I could write a fluids problem about it!
From playlist Off Topic
UNSW Photoluminescence (PL) Imaging
Photoluminescence (PL) imaging has been the most significant innovation for silicon solar cell metrology in the last decade. PL imaging relies on radiative recombination which is one of the two intrinsic recombination mechanisms in silicon (the other being Auger recombination). Radiative r
From playlist UNSW Photovoltaic Technology and Manufacturing
Craig Davidson - SpaceX and MagSail - 20th Annual International Mars Society Convention
Speaker: Craig Davidson Improving the SpaceX Mars Colonization Plans The SharkFin Magnetic Sail From the 20th Annual International Mars Society Convention, held at University of California Irvine from Sept 7-10, 2017. The four-day International Mars Society Convention brings together lea
From playlist Project Dragonfly Playlist
Twitch Talks - Image Computation for Microscopy
Presenter: Markus van Almsick Wolfram Research developers demonstrate the new features of Version 12 of the Wolfram Language that they were responsible for creating. Previously broadcast live on September 3, 2019 at twitch.tv/wolfram. For more information, visit: https://www.wolfram.com/l
From playlist Twitch Talks
MIT 7.016 Introductory Biology, Fall 2018 Instructor: Adam Martin View the complete course: https://ocw.mit.edu/7-016F18 YouTube Playlist: https://www.youtube.com/playlist?list=PLUl4u3cNGP63LmSVIVzy584-ZbjbJ-Y63 Professor Martin introduces cell imaging techniques, which are tools that all
From playlist MIT 7.016 Introductory Biology, Fall 2018
The Microscope Upgrades We've Made Along The Way | Compilation
Go to https://microcosmos.store before August 8th to get 10% off of almost everything in the store and to pick up a limited edition Dark Mode Hydra T-Shirt! This channel wouldn’t be what it is if it weren’t for one very key invention: the microscope. Everything we see, we see with the aid
From playlist Season 6
Widefield and Confocal Fluorescence Microscopy
We just learned about electron microscopy, so what was the next major innovation in microscopy in the 20th century? That would be fluorescence microscopy, of both the widefield and confocal varieties. How does this work? What is fluorescence in the first place? What are fluorophores? What
From playlist Microbiology/Infectious Diseases
Electron Microscopy (TEM and SEM)
We've talked a lot about light microscopy, but this technique has inherent limitations in resolution and magnification. The next paradigm in microscopy that emerged in the middle of the 20th century was electron microscopy. Because electrons have much shorter wavelengths than photons, elec
From playlist Microbiology/Infectious Diseases
TOP Space YouTubers Answer Your Questions | Q&A 200
This time it's different. As always, it's your space questions, but not my answers. I invited my fellow space YouTubers to give their answers to help me with the Q&A. Enjoy! 🦄 Support us on Patreon: https://patreon.com/universetoday Thanks to everyone for joining. Here are all the links
From playlist Fraser Cain / Universe Today Podcast
Mary Scott - Supervised and Unsupervised approaches for Electron Microscopy Data Analysis
Recorded 01 December 2022. Mary Scott of the University of California, Berkeley, presents "Supervised and Unsupervised approaches for Electron Microscopy Data Analysis" at IPAM's Multi-Modal Imaging with Deep Learning and Modeling Workshop. Abstract: Recently, materials science has undergo
From playlist 2022 Multi-Modal Imaging with Deep Learning and Modeling
Electron beam control in a scanning electron microscope
I describe electron beam optics and deflection as they are employed in scanning electron microscopy. http://web.jfet.org/vclk/ - Deflection amplifier http://www.johngineer.com/blog/?p=648 - CRT Christmas tree http://www.fei.com/ - FEI Company
From playlist Scanning Electron Microscope
Computational Microscopy: Utilizing Image Processing and Neural Networks
www.wolfram.com/wolfram-u/ This event features demos and tutorials using Wolfram technologies for 2D and 3D image analysis and computer vision. Wolfram's integrated workflow combines high level image processing and machine learning in one system, allowing to solve a variety of problems fr
From playlist Computational Microscopy