Super-resolution optical fluctuation imaging (SOFI) is a post-processing method for the calculation of super-resolved images from recorded image time series that is based on the temporal correlations of independently fluctuating fluorescent emitters. SOFI has been developed for super-resolution of biological specimen that are labelled with independently fluctuating fluorescent emitters (organic dyes, fluorescent proteins). In comparison to other super-resolution microscopy techniques such as STORM or PALM that rely on single-molecule localization and hence only allow one active molecule per diffraction-limited area (DLA) and timepoint, SOFI does not necessitate a controlled photoswitching and/ or photoactivation as well as long imaging times. Nevertheless, it still requires fluorophores that are cycling through two distinguishable states, either real on-/off-states or states with different fluorescence intensities. In mathematical terms SOFI-imaging relies on the calculation of cumulants, for what two distinguishable ways exist. For one thing an image can be calculated via auto-cumulants that by definition only rely on the information of each pixel itself, and for another thing an improved method utilizes the information of different pixels via the calculation of cross-cumulants. Both methods can increase the final image resolution significantly although the cumulant calculation has its limitations. Actually SOFI is able to increase the resolution in all three dimensions. (Wikipedia).
Physics - Optics: Circular Aperture - Angle of Resolution (5 of 6) Resolution of the Hubble
Visit http://ilectureonline.com for more math and science lectures! In this video I will find the resolution of the Hubble Space Telescope. Next video in series: http://youtu.be/yFFW20YnGsQ
From playlist PHYSICS 61 DIFFRACTION OF LIGHT
Carl Zeiss S-planar lens pt.2: resolution explained
In this video we take a closer look at the maximum imaging resolution of the Carl Zeiss S-Planar lens. Assuming it satisfies the Rayleigh limit, the maximum resolution of this lens should be around 723nm or 0.7um according to theory. Measurements show that the resolution is actually somewh
From playlist Carl Zeiss S-planar Photolithography Lens
Physics 59 Optical Instruments (10 of 20) Magnification of a Microscope: Exact Approach
Visit http://ilectureonline.com for more math and science lectures! To donate: http://www.ilectureonline.com/donate https://www.patreon.com/user?u=3236071 We will learn the EXACT approach to find the magnification of a microscope given the length of microscope=20cm, focal distance object
From playlist PHYSICS 59 OPTICAL INSTRUMENTS
Fluorescence optics for the analytical ultracentrifuge
I think the name says it all. More at http://www.test-tube.org.uk/
From playlist Videos about David Scott
Physics - Optics: Circular Aperture - Angle of Resolution (3 of 6) Resolution Power of the Human Eye
Visit http://ilectureonline.com for more math and science lectures! In this video I will explain the resolution ability of the human eye.. Next video in series: http://youtu.be/H2YJYFXo3yo
From playlist PHYSICS 61 DIFFRACTION OF LIGHT
A Mixture of Cold Atoms in a Structured Optical Potential by Saptarishi Chaudhuri
DISCUSSION MEETING STRUCTURED LIGHT AND SPIN-ORBIT PHOTONICS ORGANIZERS: Bimalendu Deb (IACS Kolkata, India), Tarak Nath Dey (IIT Guwahati, India), Subhasish Dutta Gupta (UOH, TIFR Hyderabad, India) and Nirmalya Ghosh (IISER Kolkata, India) DATE: 29 November 2022 to 02 December 2022 VE
From playlist Structured Light and Spin-Orbit Photonics
Phys 550 Lecture 1: Biomolecular Physics - Introduction to Biomolecular Physics
See the updated video (synced with high resolution slides) at http://nanohub.org/resources/19295 See the rest of the course, including course notes and exercises at http://nanohub.org/resources/19299 This is the first lecture in a course on biomolecular physics taught by Professors Schul
From playlist Illinois: Introduction to Biomolecular Physics - CosmoLearning.com Physics
Physics 59 Optical Instruments (9 of 20) Magnification of a Microscope: Simple Approach
Visit http://ilectureonline.com for more math and science lectures! To donate: http://www.ilectureonline.com/donate https://www.patreon.com/user?u=3236071 We will learn the SIMPLE approach to find the magnification of a microscope given the length of microscope=20cm, focal distance objec
From playlist PHYSICS 59 OPTICAL INSTRUMENTS
Seize the Moments: Enhancing Moment Estimation (...) - M. Tsang - PRACQSYS 2018 - CEB T2 2018
Mankei Tsang (Department of Electrical and Computer Engineering, National University of Singapore, Singapore & Department of Physics, National University of Singapore, Singapore) / 05.07.2018 Seize the Moments: Enhancing Moment Estimation for Subdiffraction Incoherent Imaging Inspired by
From playlist 2018 - T2 - Measurement and Control of Quantum Systems: Theory and Experiments
Photoacoustic Imaging: From Organelles to Cancer Patients / Seminar Day, Session III
Photoacoustic Imaging: From Organelles to Cancer Patients / Seminar Day, Session III Saturday, May 15, 2021 12:30 PM Using a combination of light and sound, Lihong Wang is noninvasively peering deeper inside biological tissues than previously possible. Hear about how his novel imaging t
From playlist Seminar Day
Josselin Garnier, Correlation-based imaging in random media
Josselin Garnier, Université Paris-Diderot, France Correlation-based imaging in random media
From playlist Conférence en l'honneur de Jean-Pierre Bourguignon
Frequency response test for thermal imaging sensor
I am testing the frequency response of the TPS334 IR sensor. My very crude tests agree with the datasheet, which means that the image scan time will be very long for high contrast images. Many low-cost microbolometer-based thermal imaging cameras have 80x80 sensors (6400 pixels). At a 1
From playlist Electronics
Physics - Optics: Single Slit Diffraction (5 of 15) Locations of 2nd & 3rd Max & Min = ?
Visit http://ilectureonline.com for more math and science lectures! In this video I will find all the locations of the max and min of the diffraction in general. Next video in series: http://youtu.be/vQPaAEkyDFk
From playlist PHYSICS 61 DIFFRACTION OF LIGHT
Foucault movie of a parabolic mirror F/4.2 (200 micron movement steps, very short video)
This is a video that consists of the Foucault images of the first telescope mirror that I made. It has a diameter of 8" (255mm). The mirror is an F/4.2 and the distance steps between the image frames are 200um.
From playlist old stuff
Neutron Stars and Black Holes (Lecture -13: The Realm of the Supermassive Black... by G. Srinivasan
Colloquium The Realm of the Supermassive Black Holes Speaker: G. Srinivasan (Raman Research Institute (Retired), Bangalore ) Date: Wed, 26 June 2019, 10:00 to 11:30 Venue: Madhava Lecture Hall, ICTS Campus Bangalore Abstract One of the most momentous discoveries in the history of a
From playlist Summer Course 2019: Neutron Stars and Black Holes
Physics of Membranes and their Interactions (Lecture - 3) by Ana-Suncana Smith
PROGRAM STATISTICAL BIOLOGICAL PHYSICS: FROM SINGLE MOLECULE TO CELL ORGANIZERS: Debashish Chowdhury (IIT-Kanpur, India), Ambarish Kunwar (IIT-Bombay, India) and Prabal K Maiti (IISc, India) DATE: 11 October 2022 to 22 October 2022 VENUE: Ramanujan Lecture Hall 'Fluctuation-and-noise' a
From playlist STATISTICAL BIOLOGICAL PHYSICS: FROM SINGLE MOLECULE TO CELL (2022)
Laser fundamentals II: Laser linewidth | MIT Video Demonstrations in Lasers and Optics
Laser fundamentals II: Laser linewidth Instructor: Shaoul Ezekiel View the complete course: http://ocw.mit.edu/RES-6-006S08 License: Creative Commons BY-NC-SA More information at http://ocw.mit.edu/terms More courses at http://ocw.mit.edu
From playlist MIT RES.6-006 Video Demonstrations in Lasers and Optics
Alain Aspect - Hanbury Brown - Twiss, Hong - Ou - Mandel, and other landmarks in quantum optics
Alain Aspect - Hanbury Brown - Twiss, Hong - Ou - Mandel, and other landmarks in quantum optics: from photons to atoms The second quantum revolution is based on entanglement, discovered by Einstein and Schrödinger in 1935. Its extraordinary character has been experimentally demonstrated
From playlist NOKIA-IHES Workshop
Physics - Optics: Single Slit Diffraction (8 of 15) Intensity=? General Case
Visit http://ilectureonline.com for more math and science lectures! In this video I will calculates the intensity (anywhere) in general caused by the diffraction. Next video in series: http://youtu.be/PpYCIyKJAWM
From playlist PHYSICS 61 DIFFRACTION OF LIGHT