Immune electron microscopy (more often called immunoelectron microscopy) is the equivalent of immunofluorescence, but it uses electron microscopy rather than light microscopy. Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody. This bond can form before or after embedding the cells into slides. A reaction occurs between the antigen and antibody, causing this label to become visible under the microscope. Scanning electron microscopy is a viable option if the antigen is on the surface of the cell, but transmission electron microscopy may be needed to see the label if the antigen is within the cell. (Wikipedia).
Electron Microscopy (TEM and SEM)
We've talked a lot about light microscopy, but this technique has inherent limitations in resolution and magnification. The next paradigm in microscopy that emerged in the middle of the 20th century was electron microscopy. Because electrons have much shorter wavelengths than photons, elec
From playlist Microbiology/Infectious Diseases
Electron beam control in a scanning electron microscope
I describe electron beam optics and deflection as they are employed in scanning electron microscopy. http://web.jfet.org/vclk/ - Deflection amplifier http://www.johngineer.com/blog/?p=648 - CRT Christmas tree http://www.fei.com/ - FEI Company
From playlist Scanning Electron Microscope
Types of Immune Cells Part 1: Immune Cell Function
Ok, we are ready to investigate the immune system! So where do we start? Rather than starting on the big objects, like the organs in the lymphatic system, the best way to dive in is to briefly discuss the different types of cells that comprise the immune system, and talk a little bit about
From playlist Immunology
DIY Scanning Electron Microscope - Overview
Today, I finally produced an image with my DIY scanning electron microscope. I've spent the last few months working on this project, and am encouraged by today's success. There is still a lot of work left to do in making the image higher resolution, and eliminating sources of noise, howe
From playlist Scanning Electron Microscope
Professor Jennifer Dionne: Inside out
Visualizing chemical and biological processes with nanometer-scale resolution
From playlist Materials Science and Engineering Centennial
Barrier Surfaces of the Innate Immune System
The first aspect of the innate immune system we will examine is the many barrier surfaces, such as the skin, as well as the linings of the respiratory tract and digestive tract. These cells have to constantly prevent infection from the environment, so how do they do this? We've also probab
From playlist Immunology
The World’s First MRI of a SINGLE Atom Is Here, and It Could Revolutionize Imaging
Magnetic resonance imaging is nothing new, but scientists were able to perform an MRI on a single atom. But how? » Subscribe to Seeker! http://bit.ly/subscribeseeker » Watch more Elements! http://bit.ly/ElementsPlaylist Scientists recently captured the smallest MRI ever while scanning a
From playlist Elements | Season 4 | Seeker
Science Facebook Live: Understanding SARS-CoV-2 structure informs vaccine design, clinical trials
Just weeks after the genome sequence of the recently emerged coronavirus was published online, researchers reported the cryogenic electron microscopy structure of the spike protein the virus uses to gain entry to host cells in a 19 February Science paper (https://scim.ag/2WxQ8lY). The insi
From playlist Livestreams
Proteins in 3D | The Royal Society
We are intrigued by how proteins work. Our genetic code determines the amino acid sequence of proteins, which in turn determines their 3D structure. Subscribe to our channel for exciting science videos and live events, many hosted by Brian Cox, our Professor for Public Engagement: https://
From playlist Latest talks and lectures
TEDxCaltech - Pamela Björkman - Visualizing and Engineering New Anti-HIV Agents
Pamela Björkman is the Max Delbrück Professor of Biology at Caltech, and an investigator of the Howard Hughes Medical Institute. She is well known for her pioneering work in structural studies of biological macromolecules. Her research focuses upon the structure and function of proteins of
From playlist TEDxCaltech - 1/14/11
Bacterial cell walls, antibiotics and the origins of life
How do some bacteria fly under the radar of antibiotics and our immune system? The cell wall is found in almost all bacteria and is the target for our best antibiotics. However, many bacteria can switch into L-form without a cell wall and become completely resistant to many antibiotics. In
From playlist Latest talks and lectures
Hong Zhou - New cryoEM Programs for Studying Native Biological Complexes, in situ and in Action
Recorded 15 September 2022. Hong Zhou of the University of California, Los Angeles, presents "New cryoEM Programs for Studying Native Biological Complexes, in situ and in Action" at IPAM's Computational Microscopy Tutorials. Abstract: Z. Hong Zhou1,2 1California NanoSystems Institute, Uni
From playlist Tutorials: Computational Microscopy 2022
Conformational Changes with Kate Radford
©2022 California Institute of Technology
From playlist Science Journeys
Dr. Eunsoo Kim is an assistant curator in the Division of Invertebrate Zoology at the American Museum of Natural History. Her research focuses on the evolution and ecology of single-celled eukaryotic organisms. Her long-term research goals are the investigation of the diversity and genomic
From playlist Meet the Scientists
Fluorescent microscopes are amazing!
Fluorescent microscopy was always one of my favorite parts of working with mammalian cells as it always made for spectacular images. There are a variety of techniques that make use of fluorescence microscopy, many of which are used regularly in the best labs in the world. But fluorescence
From playlist Biology and Genetics
How The Immune System ACTUALLY Works – IMMUNE
To preorder IMMUNE click here: https://kgs.link/ImmuneBook –– It’s available in English and German and at online retailers it should be available in pretty much all countries too. Sources & further reading: https://sites.google.com/view/sources-immunesystemexplained/ The human immune sys
From playlist Medicine & Biology
R1. Determining, Analyzing, and Understanding Protein Structures
MIT 5.08J Biological Chemistry II, Spring 2016 View the complete course: https://ocw.mit.edu/5-08JS16 Instructor: Shiva Mandala This recitation covers different techniques that are used to determine protein structure, as well as using the Protein Data Bank (PDB). Students then complete a
From playlist MIT 5.08J Biological Chemistry II, Spring 2016
After completing a fairly extensive survey of bacterial and viral pathogens, it's time to learn about some other more general microbiology concepts. What better place to start than the indispensable tool of the microbiologist, the microscope! What is the history of the microscope? What typ
From playlist Microbiology/Infectious Diseases
How to use a Microscope | Cells | Biology | FuseSchool
How to use a Microscope | Cells | Biology | FuseSchool If you were alive 200 years ago you would have been given some very strange advice on how to avoid getting ill. You might have been told to carry sweet smelling flowers with you or avoid going to smelly places. This was because at tha
From playlist BIOLOGY
Adventures in Super-Resolution Imaging by Daniel Coombes
Dynamics of Complex Systems - 2017 DATES: 10 May 2017 to 08 July 2017 VENUE: Madhava Lecture Hall, ICTS Bangalore This Summer Program on Dynamics of Complex Systems is second in the series. The theme for the program this year is Mathematical Biology. Over the past decades, the focus o
From playlist Dynamics of Complex Systems - 2017